PATHOLOGY OF CALCIUM TRANSPORTING SYSTEMS IN MUSCLE AND OTHER CELL TYPES
- 3 Years 2001/2004
- 134.279€ Total Award
Calcium is a universal signalling agent that controls cell function during the entire life of cells, from fertilization to death. Its final regulation within cells is performed by protein systems that transport it across the plasma membrane and across the membranes of cell organelles. The transporting systems (pumps, exchangers, channels) have different properties and the project plans to study their integrated function in living cells, including muscle cells. This will require the expression of the recombinant transporting proteins in heterologous cells, and the study of their effects on calcium homeostasis by calcium imaging techniques. Several forms of genetic pathology follow mutations in calcium transporters, especially pumps and channels. The project will study some of these genetic conditions by expressing the mutated genes. The research will also be extended to a study of the role of calcium transporting systems in the process of programmed cell death.
Scientific Publications
- 2004 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Ryanodine receptor defects in muscle genetic diseases
- 2004 BIOLOGICAL RESEARCH
Calcium signaling: A historical account
- 2003 CELL CALCIUM
Ca2+ signalling in mitochondria: mechanism and role in physiology and pathology
- 2003 JOURNAL OF BIOLOGICAL CHEMISTRY
A comparative functional analysis of plasma membrane Ca2+pump isoforms in intact cells
- 2005 JOURNAL OF BIOLOGICAL CHEMISTRY
Ca2+ signaling in HEK-293 and skeletal muscle cells expressing recombinant ryanodine receptors harboring malignant hyperthermia and central core disease mutations
- 2005 CELL CALCIUM
The Symposia on Calcium Binding Proteins and Calcium Function in Health and Disease: an historical account, and an appraisal of their role in spreading the calcium message
- 2005 BIOCHEMISTRY-MOSCOW
A historical review of cellular calcium handling, with emphasis on mitochondria
- 2002 JOURNAL OF BIOLOGICAL CHEMISTRY
Recombinant expression of the plasma membrane Na+/Ca2+ exchanger affects local and global Ca2+ homeostasis in Chinese hamster ovary cells
- 2005 FEBS Journal
The Theodor Bucher Lecture - Calcium - a universal carrier of biological signals - Delivered on 3 July 2003 at the Special FEBS Meeting in Brussels
- 2006 JOURNAL OF BIOLOGICAL CHEMISTRY
Inhibitory interaction of the plasma membrane Na+/Ca2+ exchangers with the 14-3-3 proteins